Richard D. Berlin
Center for Cell Analysis & Modeling

CCAM Microscopy Equipment

Confocal Microscopes	Widefield Microscopes
Software and Additional Equipment

 

Confocals

Zeiss LSM 780

This is a combined confocal/FCS/NLO system, mounted on an inverted Axio Observer Z1. The 780 employs the spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging, maximum sensitivity photon counting and Fluorescence Correlation Spectroscopy (FCS) as well as two adjacent PMT's. This system is equiped with the following laser lines:

Laser	Wavelengths (nm)
Diode	405 and 440
Argon	458, 488, and 514
Diode Pumped Solid State (DPSS)	561
HeNe	633
Coherent Chameleon tunable, software controlled, modelocked Ti:Sapphire laser for multi-photon excitation (NLO)	720 - 950nm

 

Zeiss LSM ConfoCor3

(Configuration)
This is a confocal and FCS system, mounted on a AxioVert 200M. This system has two avalanche photodiodes (APD) with ultimate sensitivity allowing for decreased laser power with delicate specimens. The APD's are highly efficient in the red wavelength range where fluorescent signal can be very weak.This system is equipped with the following laser lines:

Laser	Wavelengths (nm)
Argon	458, 477, 488, and 514
Diode Pumped Solid State (DPSS)	561
HeNe	633

Zeiss LSM 510 Meta

(Configuration)

The Meta is mounted on an Axiovert 200M with automated xyz control. The system is equipped with the specialized Meta detector as well as 2 traditional photomultiplier tubes and a transmitted light detector. Laser excitation lines include:

Laser	Wavelengths (nm)
Argon	458, 488, and 514
HeNe	543
HeNe	633

Perkin Elmer UltraView

Spinning Disk Confocal is mounted on an inverted, Nikon TE2000 microscope. This system employs the Micropoint Mosaic system for photomanipulation techniques such as FRAP or Photoactivation. It is capable of exciting with the following laser lines:

Laser	Wavelengths (nm)
Diode	442
Argon	488 and 514
Argon	568 and 647

Widefield Microscopes

Zeiss Total Internal Reflection Fluorescence (TIRF)

Imaging system including 100 mW argon laser with 458, 488 and 514 nm excitation, Axio Observer fully motorize microscope and Axiocam MRM camera controlled by AxioVision software.

Software and Additional Equipment

Imaris Surpass 3-D rendering and analysis software from Bitplane.

Molecular Devices' MetaMorph;software for analysis, image processing as well as acquisition and device control.

Canon CanoScan 8400F flat bed scanner

Hitachi's R-1200 Rapid Scan 60Mhz permanent magnet NMR Spectrometer

Spex Fluorolog single excitation, dual emission spectrofluorimeter.

Additional equipment and facilities are also available in the laboratories of the CCAM faculty.